Baculovirusses

Immunogenicity Assessment of Rift Valley Fever Virus Virus-Like Particles in BALB/c Mice.

Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV) and is spread by arthropod vectors. Virus-like particle (VLP) vaccines, which have the advantages of strong immunogenicity and safety, play an important role in the prevention of this disease. VLPs for RVFV were successfully prepared by our research group using a baculovirus-insect cell expression system. To study the immunogenicity of these RVFV VLPs, a correct 3rd or 4th generation recombinant baculovirus, rBac-N-G, was identified and used to infect Sf9 cells, which were cultured in suspension at a large scale. Subsequently, cell debris was removed by centrifugation, and the VLPs were concentrated by ultracentrifugation and purified using a sucrose gradient, after which they were used to immunize BALB/c mice by intramuscular injection. The results showed that the RVFV VLPs prepared by our research group could effectively induce mice to produce RVFV neutralizing antibodies and that the prepared VLPs could stimulate mouse spleen cells to produce high levels of the cytokines IL-4 and IFN-γ.

Moreover, the proportion of lymphocytes producing IL-4 and IFN-γ in the spleen of mice immunized with RVFV VLPs was significantly increased. Therefore, the RVFV VLPs prepared in this study had strong immunogenicity and could effectively activate humoral and cellular immunity in mice. This study lays a solid foundation for the development of RVFV VLP vaccine candidates and promotes the healthy development of animal husbandry and human public health.


Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System.

Integral membrane proteins have a critical role in fundamental biological processes; they are major drug targets and therefore of high research interest. Recombinant protein production is the first step in the protein tool generation for biochemical and biophysical studies.

Here, we provide simplified protocols that facilitate the generation of high-quality virus and initial expression analysis for integral membrane protein targets utilizing the baculovirus-mediated expression system in insect cells. The protocol steps include generation of viruses, virus quality control.

Baculovirus penaei

Oneq-V285-OneqV285100D Oneq-V285-100D
EUR 515

Baculovirus penaei

Oneq-V285-OneqV285150D Oneq-V285-150D
EUR 594

Baculovirus penaei

Oneq-V285-OneqV28550D Oneq-V285-50D
EUR 413

Baculovirus penaei

PCR-V285-PCRV28548D PCR-V285-48D
EUR 230

Baculovirus penaei

PCR-V285-PCRV28596D PCR-V285-96D
EUR 312

Monodon baculovirus

Oneq-V280-OneqV280100D Oneq-V280-100D
EUR 515

Monodon baculovirus

Oneq-V280-OneqV280150D Oneq-V280-150D
EUR 594

Monodon baculovirus

Oneq-V280-OneqV28050D Oneq-V280-50D
EUR 413

Monodon baculovirus

PCR-V280-PCRV28048D PCR-V280-48D
EUR 230

Monodon baculovirus

PCR-V280-PCRV28096D PCR-V280-96D
EUR 312

IL12 p40 Baculovirus

cyt-134 2µg
EUR 60
Description: Recombinant Human Interleukin-12 p40, Baculovirus

Sapphire Baculovirus DNA

ABP-BVD-10001 15 µg (10 rxns) Ask for price

Baculovirus penaei PCR kit

PCR-V285-48D 50T
EUR 543.6

Baculovirus penaei PCR kit

PCR-V285-96D 100T
EUR 686.4

Monodon baculovirus PCR kit

PCR-V280-48D 50T
EUR 543.6

Monodon baculovirus PCR kit

PCR-V280-96D 100T
EUR 686.4

Baculovirus penaei RT PCR kit

RTq-V285-100D 100T
EUR 860.4

Baculovirus penaei RT PCR kit

RTq-V285-150D 150T
EUR 969.6

Baculovirus penaei RT PCR kit

RTq-V285-50D 50T
EUR 717.6

Monodon baculovirus RT PCR kit

RTq-V280-100D 100T
EUR 860.4

Monodon baculovirus RT PCR kit

RTq-V280-150D 150T
EUR 969.6

Monodon baculovirus RT PCR kit

RTq-V280-50D 50T
EUR 717.6

NC (negative control baculovirus)

C13 1 ml
EUR 195
Description: Protein expression

Green Kit. Baculovirus GFP vector.

K20 1 Kit
EUR 695
Description: Protein expression

Controls Kit. Baculovirus controls.

K26 1 Kit
EUR 295
Description: Protein expression

Non-occluded enteric baculovirus

Oneq-V298-OneqV298100R Oneq-V298-100R
EUR 1028

Non-occluded enteric baculovirus

Oneq-V298-OneqV298150R Oneq-V298-150R
EUR 1438

Non-occluded enteric baculovirus

Oneq-V298-OneqV29850R Oneq-V298-50R
EUR 728

Non-occluded enteric baculovirus

PCR-V298-PCRV29848R PCR-V298-48R
EUR 485

Non-occluded enteric baculovirus

PCR-V298-PCRV29896R PCR-V298-96R
EUR 685

Recombinant Human Decorin (Baculovirus)

CS86-10ug 10ug
EUR 120
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Decorin (Baculovirus)

CS86-1mg 1mg
EUR 1094.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Decorin (Baculovirus)

CS86-500ug 500ug
EUR 790.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Decorin (Baculovirus)

CS86-50ug 50ug
EUR 242.4
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Baculovirus penaei One-Step PCR kit

Oneq-V285-100D 100T
EUR 1039.2

Baculovirus penaei One-Step PCR kit

Oneq-V285-150D 150T
EUR 1177.2

Baculovirus penaei One-Step PCR kit

Oneq-V285-50D 50T
EUR 861.6

ProGreen. Baculovirus GFP marker vector.

A1 25 ul
EUR 420
Description: Protein expression

Monodon baculovirus One-Step PCR kit

Oneq-V280-100D 100T
EUR 1039.2

Monodon baculovirus One-Step PCR kit

Oneq-V280-150D 150T
EUR 1177.2

Monodon baculovirus One-Step PCR kit

Oneq-V280-50D 50T
EUR 861.6

pVL1393. General baculovirus plasmid vector.

B1 50 ul
EUR 340
Description: Protein expression

Non-occluded enteric baculovirus PCR kit

PCR-V298-48R 50T
EUR 987.6

Non-occluded enteric baculovirus PCR kit

PCR-V298-96R 100T
EUR 1335.6

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