Baculovirusses

Immunogenicity Assessment of Rift Valley Fever Virus Virus-Like Particles in BALB/c Mice.

Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV) and is spread by arthropod vectors. Virus-like particle (VLP) vaccines, which have the advantages of strong immunogenicity and safety, play an important role in the prevention of this disease. VLPs for RVFV were successfully prepared by our research group using a baculovirus-insect cell expression system. To study the immunogenicity of these RVFV VLPs, a correct 3rd or 4th generation recombinant baculovirus, rBac-N-G, was identified and used to infect Sf9 cells, which were cultured in suspension at a large scale. Subsequently, cell debris was removed by centrifugation, and the VLPs were concentrated by ultracentrifugation and purified using a sucrose gradient, after which they were used to immunize BALB/c mice by intramuscular injection. The results showed that the RVFV VLPs prepared by our research group could effectively induce mice to produce RVFV neutralizing antibodies and that the prepared VLPs could stimulate mouse spleen cells to produce high levels of the cytokines IL-4 and IFN-γ.

Moreover, the proportion of lymphocytes producing IL-4 and IFN-γ in the spleen of mice immunized with RVFV VLPs was significantly increased. Therefore, the RVFV VLPs prepared in this study had strong immunogenicity and could effectively activate humoral and cellular immunity in mice. This study lays a solid foundation for the development of RVFV VLP vaccine candidates and promotes the healthy development of animal husbandry and human public health.


Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System.

Integral membrane proteins have a critical role in fundamental biological processes; they are major drug targets and therefore of high research interest. Recombinant protein production is the first step in the protein tool generation for biochemical and biophysical studies.

Here, we provide simplified protocols that facilitate the generation of high-quality virus and initial expression analysis for integral membrane protein targets utilizing the baculovirus-mediated expression system in insect cells. The protocol steps include generation of viruses, virus quality control.

Monodon baculovirus PCR kit

PCR-V280-48D 50T
EUR 425.80
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Monodon baculovirus

Monodon baculovirus PCR kit

PCR-V280-96D 100T
EUR 521.50
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Monodon baculovirus

Baculovirus penaei PCR kit

PCR-V285-48D 50T
EUR 425.80
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Baculovirus penaei

Baculovirus penaei PCR kit

PCR-V285-96D 100T
EUR 521.50
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Baculovirus penaei

Monodon baculovirus RT PCR kit

RTq-V280-100D 100T
EUR 628.50
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Monodon baculovirus .

Monodon baculovirus RT PCR kit

RTq-V280-150D 150T
EUR 701.00
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Monodon baculovirus .

Monodon baculovirus RT PCR kit

RTq-V280-50D 50T
EUR 532.80
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Monodon baculovirus .

Baculovirus penaei RT PCR kit

RTq-V285-100D 100T
EUR 628.50
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Baculovirus penaei .

Baculovirus penaei RT PCR kit

RTq-V285-150D 150T
EUR 701.00
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Baculovirus penaei .

Baculovirus penaei RT PCR kit

RTq-V285-50D 50T
EUR 532.80
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Baculovirus penaei .

Macrophage Colony Stimulating Factor, Baculovirus Protein

20-abx262208
  • EUR 328.00
  • EUR 5826.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Sapphire Baculovirus DNA and Transfection Kit

ABP-BVD-10002 1 kit Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Baculorvirus DNA

Monodon baculovirus One-Step PCR kit

Oneq-V280-100D 100T
EUR 747.40
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Monodon baculovirus.

Monodon baculovirus One-Step PCR kit

Oneq-V280-150D 150T
EUR 838.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Monodon baculovirus.

Monodon baculovirus One-Step PCR kit

Oneq-V280-50D 50T
EUR 628.50
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Monodon baculovirus.

Baculovirus penaei One-Step PCR kit

Oneq-V285-100D 100T
EUR 747.40
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Baculovirus penaei.

Baculovirus penaei One-Step PCR kit

Oneq-V285-150D 150T
EUR 838.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Baculovirus penaei.

Baculovirus penaei One-Step PCR kit

Oneq-V285-50D 50T
EUR 628.50
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Baculovirus penaei.

Non-occluded enteric baculovirus PCR kit

PCR-V298-48R 50T
EUR 524.40
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Non-occluded enteric baculovirus

Non-occluded enteric baculovirus PCR kit

PCR-V298-96R 100T
EUR 669.40
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Non-occluded enteric baculovirus

Non-occluded enteric baculovirus RT PCR kit

RTq-V298-100R 100T
EUR 776.40
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Non-occluded enteric baculovirus .

Non-occluded enteric baculovirus RT PCR kit

RTq-V298-150R 150T
EUR 877.90
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Non-occluded enteric baculovirus .

Non-occluded enteric baculovirus RT PCR kit

RTq-V298-50R 50T
EUR 631.40
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Non-occluded enteric baculovirus .

Non-occluded enteric baculovirus One-Step PCR kit

Oneq-V298-100R 100T
EUR 933.00
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Non-occluded enteric baculovirus.

Non-occluded enteric baculovirus One-Step PCR kit

Oneq-V298-150R 150T
EUR 1060.60
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Non-occluded enteric baculovirus.

Non-occluded enteric baculovirus One-Step PCR kit

Oneq-V298-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Non-occluded enteric baculovirus.

Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins (Sf9-HCP, soluble fraction)

730-145-S15 1 mg
EUR 847.00

Recombinant 2019-nCoV coronavirus Spike protein , S1 subunit, expressed in Baculovirus-Insect cells

Spike-195V 100ug
EUR 1610.00
  • Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at +4 degrees Celsius before opening to recover the entire contents.
Description: Recombinant COVID-19 (2019 novel coronavirus) Spike protein S1 subunit was fused to His-tag at C-terminus and expressed in Baculovirus-Insect cells. The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell.S1 mainly contains a receptor binding domain (RBD) and recognize the cell surface receptor. S2 essential for membrane fusion. S protein are important for neutralizing-antibody and T-cell responses, as well as protective immunity.

Recombinant 2019-nCoV coronavirus Spike protein ,S2 ECD, expressed in Baculovirus-Insect cells

Spike-197V 100ug
EUR 1610.00
  • Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at +4 degrees Celsius before opening to recover the entire contents.
Description: Recombinant COVID-19 (2019 novel coronavirus) Spike protein (S2 ECD) was fused to His-tag at C-terminus and expressed in Baculovirus-Insect cells. The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell.S1 mainly contains a receptor binding domain (RBD) and recognize the cell surface receptor. S2 essential for membrane fusion. S protein are important for neutralizing-antibody and T-cell responses, as well as protective immunity.

Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins (Sf9-HCP, soluble fraction) positive control (10 ug/ml)

730-145-PC1 1 ml
EUR 225.00

Rabbit anti-Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins (HCPs) IgG (Low molecular weight fraction, soluble fraction affinity pure)

730-145-11A 100 ug
EUR 457.00

Rabbit anti-Spodoptera frugiperda/Baculovirus (Sf9) Host Cell Proteins IgG (HCPs) (High molecular weight fraction, soluble fraction, affinity pure)

730-145-12A 100 ug
EUR 457.00

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