Baculovirusses

Immunogenicity Assessment of Rift Valley Fever Virus Virus-Like Particles in BALB/c Mice.

Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV) and is spread by arthropod vectors. Virus-like particle (VLP) vaccines, which have the advantages of strong immunogenicity and safety, play an important role in the prevention of this disease. VLPs for RVFV were successfully prepared by our research group using a baculovirus-insect cell expression system. To study the immunogenicity of these RVFV VLPs, a correct 3rd or 4th generation recombinant baculovirus, rBac-N-G, was identified and used to infect Sf9 cells, which were cultured in suspension at a large scale. Subsequently, cell debris was removed by centrifugation, and the VLPs were concentrated by ultracentrifugation and purified using a sucrose gradient, after which they were used to immunize BALB/c mice by intramuscular injection. The results showed that the RVFV VLPs prepared by our research group could effectively induce mice to produce RVFV neutralizing antibodies and that the prepared VLPs could stimulate mouse spleen cells to produce high levels of the cytokines IL-4 and IFN-γ.

Moreover, the proportion of lymphocytes producing IL-4 and IFN-γ in the spleen of mice immunized with RVFV VLPs was significantly increased. Therefore, the RVFV VLPs prepared in this study had strong immunogenicity and could effectively activate humoral and cellular immunity in mice. This study lays a solid foundation for the development of RVFV VLP vaccine candidates and promotes the healthy development of animal husbandry and human public health.


Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System.

Integral membrane proteins have a critical role in fundamental biological processes; they are major drug targets and therefore of high research interest. Recombinant protein production is the first step in the protein tool generation for biochemical and biophysical studies.

Here, we provide simplified protocols that facilitate the generation of high-quality virus and initial expression analysis for integral membrane protein targets utilizing the baculovirus-mediated expression system in insect cells. The protocol steps include generation of viruses, virus quality control.

Monodon baculovirus PCR kit

PCR-V280-96D 100T
EUR 572

Baculovirus penaei PCR kit

PCR-V285-48D 50T
EUR 453

Baculovirus penaei PCR kit

PCR-V285-96D 100T
EUR 572

Recombinant Human Decorin (Baculovirus)

CS86-10ug 10ug
EUR 100
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Decorin (Baculovirus)

CS86-1mg 1mg
EUR 912
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Decorin (Baculovirus)

CS86-500ug 500ug
EUR 659
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human Decorin (Baculovirus)

CS86-50ug 50ug
EUR 202
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Monodon baculovirus RT PCR kit

RTq-V280-100D 100T
EUR 717

Monodon baculovirus RT PCR kit

RTq-V280-150D 150T
EUR 808

Monodon baculovirus RT PCR kit

RTq-V280-50D 50T
EUR 598

Baculovirus penaei RT PCR kit

RTq-V285-100D 100T
EUR 717

Baculovirus penaei RT PCR kit

RTq-V285-150D 150T
EUR 808

Baculovirus penaei RT PCR kit

RTq-V285-50D 50T
EUR 598

Monodon baculovirus One-Step PCR kit

Oneq-V280-100D 100T
EUR 866

Monodon baculovirus One-Step PCR kit

Oneq-V280-150D 150T
EUR 981

Monodon baculovirus One-Step PCR kit

Oneq-V280-50D 50T
EUR 718

Baculovirus penaei One-Step PCR kit

Oneq-V285-100D 100T
EUR 866

Baculovirus penaei One-Step PCR kit

Oneq-V285-150D 150T
EUR 981

Baculovirus penaei One-Step PCR kit

Oneq-V285-50D 50T
EUR 718

Non-occluded enteric baculovirus PCR kit

PCR-V298-48R 50T
EUR 823

Non-occluded enteric baculovirus PCR kit

PCR-V298-96R 100T
EUR 1113

Recombinant Plasmodium Berghei CG10 Protein (Baculovirus)

VAng-Wyb7596-inquire inquire Ask for price
Description: Plasmodium Berghei Putative chloroquine resistance transporter protein, partial, recombinant protein.

Recombinant Plasmodium Berghei COI Protein (Baculovirus)

VAng-Wyb7600-inquire inquire Ask for price
Description: Plasmodium Berghei Cytochrome c oxidase subunit 1 protein, partial, recombinant protein.

Recombinant Plasmodium Berghei CPK4 Protein (Baculovirus)

VAng-Wyb7604-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) Calcium-dependent protein kinase 4 protein, partial, recombinant protein.

Recombinant Plasmodium Berghei CS Protein (Baculovirus)

VAng-Wyb7616-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) Circumsporozoite protein, partial, recombinant protein.

Recombinant Plasmodium Berghei PB000816.02.0 Protein (Baculovirus)

VAng-Wyb7660-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein.

Recombinant Plasmodium Chabaudi CG10 Protein (Baculovirus)

VAng-Wyb7708-inquire inquire Ask for price
Description: Plasmodium Chabaudi Putative chloroquine resistance transporter protein, partial, recombinant protein.

Recombinant Plasmodium Chabaudi COI Protein (Baculovirus)

VAng-Wyb7712-inquire inquire Ask for price
Description: Plasmodium Chabaudi Cytochrome c oxidase subunit 1 protein, partial, recombinant protein.

Recombinant Plasmodium Chabaudi PC000176.04.0 Protein (Baculovirus)

VAng-Wyb7736-inquire inquire Ask for price
Description: Plasmodium Chabaudi Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial protein, recombinant protein.

Recombinant Plasmodium Chabaudi PC000321.02.0 Protein (Baculovirus)

VAng-Wyb7740-inquire inquire Ask for price
Description: Plasmodium Chabaudi Protein SEY1 homolog, partial, recombinant protein.

Recombinant Plasmodium Yoelii CPK1 Protein (Baculovirus)

VAng-Wyb7895-inquire inquire Ask for price
Description: Plasmodium Yoelii Calcium-dependent protein kinase 1 protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii CPK3 Protein (Baculovirus)

VAng-Wyb7899-inquire inquire Ask for price
Description: Plasmodium Yoelii Calcium-dependent protein kinase 3 protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii CPK4 Protein (Baculovirus)

VAng-Wyb7903-inquire inquire Ask for price
Description: Plasmodium Yoelii Calcium-dependent protein kinase 4 protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY01232 Protein (Baculovirus)

VAng-Wyb7947-inquire inquire Ask for price
Description: Plasmodium Yoelii Myosin-A protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY03426 Protein (Baculovirus)

VAng-Wyb7979-inquire inquire Ask for price
Description: Plasmodium Yoelii Translation factor GUF1 homolog, mitochondrial protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY03776 Protein (Baculovirus)

VAng-Wyb7983-inquire inquire Ask for price
Description: Plasmodium Yoelii tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY05061 Protein (Baculovirus)

VAng-Wyb7987-inquire inquire Ask for price
Description: Plasmodium Yoelii Putative chloroquine resistance transporter protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY05764 Protein (Baculovirus)

VAng-Wyb7991-inquire inquire Ask for price
Description: Plasmodium Yoelii Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY07549 Protein (Baculovirus)

VAng-Wyb7999-inquire inquire Ask for price
Description: Plasmodium Yoelii Pescadillo homolog protein, partial, recombinant protein.

Recombinant Plasmodium Falciparum GARP Protein (Baculovirus)

VAng-Wyb6440-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate FC27 / Papua New Guinea) Glutamic acid-rich protein, partial, recombinant protein.

Recombinant Plasmodium Falciparum GGI.R1 Protein (Baculovirus)

VAng-Wyb6448-inquire inquire Ask for price
Description: Plasmodium Falciparum Glucose-6-phosphate isomerase, partial, recombinant protein.

Recombinant Plasmodium Falciparum HSP70 Protein (Baculovirus)

VAng-Wyb6503-inquire inquire Ask for price
Description: Plasmodium Falciparum Heat shock 70 kDa protein, partial, recombinant protein.

Recombinant Plasmodium Falciparum PFS230 Protein (Baculovirus)

VAng-Wyb6734-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Gametocyte surface protein P230, partial, recombinant protein.

Recombinant Plasmodium Falciparum POLD1 Protein (Baculovirus)

VAng-Wyb6748-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate K1 / Thailand) DNA polymerase delta catalytic subunit, partial, recombinant protein.

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