Immunogenicity Assessment of Rift Valley Fever Virus Virus-Like Particles in BALB/c Mice.
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV) and is spread by arthropod vectors. Virus-like particle (VLP) vaccines, which have the advantages of strong immunogenicity and safety, play an important role in the prevention of this disease. VLPs for RVFV were successfully prepared by our research group using a baculovirus-insect cell expression system. To study the immunogenicity of these RVFV VLPs, a correct 3rd or 4th generation recombinant baculovirus, rBac-N-G, was identified and used to infect Sf9 cells, which were cultured in suspension at a large scale. Subsequently, cell debris was removed by centrifugation, and the VLPs were concentrated by ultracentrifugation and purified using a sucrose gradient, after which they were used to immunize BALB/c mice by intramuscular injection. The results showed that the RVFV VLPs prepared by our research group could effectively induce mice to produce RVFV neutralizing antibodies and that the prepared VLPs could stimulate mouse spleen cells to produce high levels of the cytokines IL-4 and IFN-γ.
Moreover, the proportion of lymphocytes producing IL-4 and IFN-γ in the spleen of mice immunized with RVFV VLPs was significantly increased. Therefore, the RVFV VLPs prepared in this study had strong immunogenicity and could effectively activate humoral and cellular immunity in mice. This study lays a solid foundation for the development of RVFV VLP vaccine candidates and promotes the healthy development of animal husbandry and human public health.
Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System.
Integral membrane proteins have a critical role in fundamental biological processes; they are major drug targets and therefore of high research interest. Recombinant protein production is the first step in the protein tool generation for biochemical and biophysical studies.
Here, we provide simplified protocols that facilitate the generation of high-quality virus and initial expression analysis for integral membrane protein targets utilizing the baculovirus-mediated expression system in insect cells. The protocol steps include generation of viruses, virus quality control.
) Recombinant Human Decorin (Baculovirus) |
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| CS86-10ug | Novoprotein | 10ug | EUR 100 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
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Recombinant Human Decorin (Baculovirus) |
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| CS86-1mg | Novoprotein | 1mg | EUR 912 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
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Recombinant Human Decorin (Baculovirus) |
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| CS86-500ug | Novoprotein | 500ug | EUR 659 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
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Recombinant Human Decorin (Baculovirus) |
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| CS86-50ug | Novoprotein | 50ug | EUR 202 |
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Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
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 Monodon baculovirus PCR kit |
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| PCR-V280-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Monodon baculovirus |
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Monodon baculovirus PCR kit |
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| PCR-V280-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Monodon baculovirus |
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 Baculovirus penaei PCR kit |
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| PCR-V285-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Baculovirus penaei |
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Baculovirus penaei PCR kit |
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| PCR-V285-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Baculovirus penaei |
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 Monodon baculovirus RT PCR kit |
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| RTq-V280-100D | Bioingentech | 100T | EUR 628.5 |
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Description: A Real-Time PCR kit for detection of Monodon baculovirus . |
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Monodon baculovirus RT PCR kit |
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| RTq-V280-150D | Bioingentech | 150T | EUR 701 |
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Description: A Real-Time PCR kit for detection of Monodon baculovirus . |
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Monodon baculovirus RT PCR kit |
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| RTq-V280-50D | Bioingentech | 50T | EUR 532.8 |
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Description: A Real-Time PCR kit for detection of Monodon baculovirus . |
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 Baculovirus penaei RT PCR kit |
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| RTq-V285-100D | Bioingentech | 100T | EUR 628.5 |
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Description: A Real-Time PCR kit for detection of Baculovirus penaei . |
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Baculovirus penaei RT PCR kit |
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| RTq-V285-150D | Bioingentech | 150T | EUR 701 |
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Description: A Real-Time PCR kit for detection of Baculovirus penaei . |
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Baculovirus penaei RT PCR kit |
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| RTq-V285-50D | Bioingentech | 50T | EUR 532.8 |
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Description: A Real-Time PCR kit for detection of Baculovirus penaei . |
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 Sapphire Baculovirus DNA and Transfection Kit |
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| ABP-BVD-10002 | Allele Biotech | 1 kit | Ask for price |
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 Macrophage Colony Stimulating Factor, Baculovirus Protein |
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| 20-abx262208 | Abbexa |
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 Baculovirus expression vector pAc-g-CH3 |
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| 30R-AB001 | Fitzgerald | 5 ug | EUR 616 |
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Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant. |
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) Baculovirus Envelope gp64 protein antibody (PE) |
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| 61R-1406 | Fitzgerald | 100 ug | EUR 435 |
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Description: Mouse monoclonal baculovirus envelope gp64 protein antibody (PE) |
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 Monodon baculovirus One-Step PCR kit |
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| Oneq-V280-100D | Bioingentech | 100T | EUR 747.4 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Monodon baculovirus. |
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Monodon baculovirus One-Step PCR kit |
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| Oneq-V280-150D | Bioingentech | 150T | EUR 838.75 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Monodon baculovirus. |
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Monodon baculovirus One-Step PCR kit |
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| Oneq-V280-50D | Bioingentech | 50T | EUR 628.5 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Monodon baculovirus. |
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 Baculovirus penaei One-Step PCR kit |
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| Oneq-V285-100D | Bioingentech | 100T | EUR 747.4 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Baculovirus penaei. |
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Baculovirus penaei One-Step PCR kit |
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| Oneq-V285-150D | Bioingentech | 150T | EUR 838.75 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Baculovirus penaei. |
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Baculovirus penaei One-Step PCR kit |
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| Oneq-V285-50D | Bioingentech | 50T | EUR 628.5 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Baculovirus penaei. |
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 Non-occluded enteric baculovirus PCR kit |
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| PCR-V298-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Non-occluded enteric baculovirus |
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Non-occluded enteric baculovirus PCR kit |
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| PCR-V298-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Non-occluded enteric baculovirus |
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) Recombinant Human Adiponectin Protein (Baculovirus cells) |
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| VAng-2891Lsx-50g | Creative Biolabs | 50 µg | EUR 3404 |
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Description: Recombinant Human Adiponectin is expressed in Sf9. (Uniprot ID: Q15848) |
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 (Baculovirus)) Recombinant HCV Protein (Full Length) (Baculovirus) |
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| VAng-Lsx0203-inquire | Creative Biolabs | inquire | Ask for price |
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Description: HCV (full length), recombinant protein from Baculovirus. |
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) Recombinant Plasmodium Berghei CG10 Protein (Baculovirus) |
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| VAng-Wyb7596-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Berghei Putative chloroquine resistance transporter protein, partial, recombinant protein. |
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) Recombinant Plasmodium Berghei COI Protein (Baculovirus) |
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| VAng-Wyb7600-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Berghei Cytochrome c oxidase subunit 1 protein, partial, recombinant protein. |
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) Recombinant Plasmodium Berghei CPK4 Protein (Baculovirus) |
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| VAng-Wyb7604-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Berghei (strain Anka) Calcium-dependent protein kinase 4 protein, partial, recombinant protein. |
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) Recombinant Plasmodium Berghei CS Protein (Baculovirus) |
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| VAng-Wyb7616-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Berghei (strain Anka) Circumsporozoite protein, partial, recombinant protein. |
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) Recombinant Plasmodium Berghei PB000816.02.0 Protein (Baculovirus) |
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| VAng-Wyb7660-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Berghei (strain Anka) tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein. |
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) Recombinant Plasmodium Chabaudi CG10 Protein (Baculovirus) |
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| VAng-Wyb7708-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Chabaudi Putative chloroquine resistance transporter protein, partial, recombinant protein. |
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) Recombinant Plasmodium Chabaudi COI Protein (Baculovirus) |
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| VAng-Wyb7712-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Chabaudi Cytochrome c oxidase subunit 1 protein, partial, recombinant protein. |
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) Recombinant Plasmodium Chabaudi PC000176.04.0 Protein (Baculovirus) |
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| VAng-Wyb7736-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Chabaudi Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial protein, recombinant protein. |
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) Recombinant Plasmodium Chabaudi PC000321.02.0 Protein (Baculovirus) |
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| VAng-Wyb7740-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Chabaudi Protein SEY1 homolog, partial, recombinant protein. |
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) Recombinant Plasmodium Yoelii CPK1 Protein (Baculovirus) |
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| VAng-Wyb7895-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Yoelii Calcium-dependent protein kinase 1 protein, partial, recombinant protein. |
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) Recombinant Plasmodium Yoelii CPK3 Protein (Baculovirus) |
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| VAng-Wyb7899-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Yoelii Calcium-dependent protein kinase 3 protein, partial, recombinant protein. |
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) Recombinant Plasmodium Yoelii CPK4 Protein (Baculovirus) |
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| VAng-Wyb7903-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Yoelii Calcium-dependent protein kinase 4 protein, partial, recombinant protein. |
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) Recombinant Plasmodium Yoelii PY01232 Protein (Baculovirus) |
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| VAng-Wyb7947-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Yoelii Myosin-A protein, partial, recombinant protein. |
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) Recombinant Plasmodium Yoelii PY03426 Protein (Baculovirus) |
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| VAng-Wyb7979-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Yoelii Translation factor GUF1 homolog, mitochondrial protein, partial, recombinant protein. |
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) Recombinant Plasmodium Yoelii PY03776 Protein (Baculovirus) |
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| VAng-Wyb7983-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Yoelii tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein. |
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) Recombinant Plasmodium Yoelii PY05061 Protein (Baculovirus) |
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| VAng-Wyb7987-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Plasmodium Yoelii Putative chloroquine resistance transporter protein, partial, recombinant protein. |
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